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Developmental Biology

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JoVE Journal Developmental Biology
Translating Ribosome Affinity Purification (TRAP) to Investigate Arabidopsis thaliana Root Development at a Cell Type-Specific Scale
 

Translating Ribosome Affinity Purification (TRAP) to Investigate Arabidopsis thaliana Root Development at a Cell Type-Specific Scale

Article DOI: 10.3791/60919-v • 09:41 min • May 14th, 2020
May 14th, 2020 •
1, 2, 1,3
1Department of Plant and Microbial Biology, University of Zurich, 2Biophore - UNIL, 3Laboratory of Cell and Molecular Biology, Institute of Biology, University of Neuchâtel

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Translating ribosome affinity purification (TRAP) offers the possibility to dissect developmental programs with minimal processing of organs and tissues. The protocol yields high-quality RNA from cells targeted with a green fluorescent protein (GFP)-labeled ribosomal subunit. Downstream analysis tools, such as qRT-PCR or RNA-seq, reveal tissue and cell type-specific expression profiles.

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Translating Ribosome Affinity Purification TRAP Arabidopsis Thaliana Root Development Cell Type-specific Scale Cell-type-specific Promoters Tagged Ribosomal Sub-unit Isolate Polysome RNA Ultra-low Input Methods High Quality RNA Libraries Plant Research Cell Wall-related Signaling Pathways Mechanical Signaling Pathways Cell-cell Communication Lateral Root Formation Step-by-step Video Instruction Sterilization Of Homozygous Lines Gas Sterilization Imbibed Seed Mix Square Petri Dish Lids
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