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Identificatie van Neutrophil Extracellular Traps in Paraffine-Embedded Feline Arterial Thrombi using Immunofluorescentie Microscopie
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Identificatie van Neutrophil Extracellular Traps in Paraffine-Embedded Feline Arterial Thrombi using Immunofluorescentie Microscopie
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March 29, 2020
DOI:
1Veterinary Medical Teaching Hospital, School of Veterinary Medicine,University of California, Davis, 2Department of Veterinary Surgical and Radiological Sciences, School of Veterinary Medicine,University of California, Davis, 3Department of Veterinary Medicine and Epidemiology, School of Veterinary Medicine,University of California, Davis
Summary
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We beschrijven een methode om neutrofiele extracellulaire vallen (NET's) te identificeren in formaldehyde-vaste en paraffine-ingebedde katachtige cardiogene trombi met behulp van warmte-geïnduceerde antigen retrieval en een dubbele immunolabeling protocol.
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Duler, L., Nguyen, N., Ontiveros, E., Li, R. H. L. Identification of Neutrophil Extracellular Traps in Paraffin-Embedded Feline Arterial Thrombi using Immunofluorescence Microscopy. J. Vis. Exp. (157), e60834, doi:10.3791/60834 (2020).
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