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Immunology and Infection

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JoVE Journal Immunology and Infection
Identification of Neutrophil Extracellular Traps in Paraffin-Embedded Feline Arterial Thrombi using Immunofluorescence Microscopy
 

Identification of Neutrophil Extracellular Traps in Paraffin-Embedded Feline Arterial Thrombi using Immunofluorescence Microscopy

Article DOI: 10.3791/60834-v • 08:48 min • March 29th, 2020
March 29th, 2020 •
1, 2, 3, 2
1Veterinary Medical Teaching Hospital, School of Veterinary Medicine, University of California, Davis, 2Department of Veterinary Surgical and Radiological Sciences, School of Veterinary Medicine, University of California, Davis, 3Department of Veterinary Medicine and Epidemiology, School of Veterinary Medicine, University of California, Davis

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We describe a method to identify neutrophil extracellular traps (NETs) in formaldehyde-fixed and paraffin-embedded feline cardiogenic arterial thrombi using heat-induced antigen retrieval and a double immunolabeling protocol.

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Neutrophil Extracellular Traps Paraffin-embedded Immunofluorescence Microscopy Feline Arterial Thrombi Antigen Retrieval Histological Stains DNA Detection Extracellular Proteins Thrombosis In Cats Veterinary Species Deparaffinization Rehydration Glass Slides Saline Ethanol Concentration TBST Treatment
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