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Identification of Neutrophil Extracellular Traps in Paraffin-Embedded Feline Arterial Thrombi using Immunofluorescence Microscopy
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Identification of Neutrophil Extracellular Traps in Paraffin-Embedded Feline Arterial Thrombi using Immunofluorescence Microscopy
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March 29, 2020
DOI:
1Veterinary Medical Teaching Hospital, School of Veterinary Medicine,University of California, Davis, 2Department of Veterinary Surgical and Radiological Sciences, School of Veterinary Medicine,University of California, Davis, 3Department of Veterinary Medicine and Epidemiology, School of Veterinary Medicine,University of California, Davis
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We describe a method to identify neutrophil extracellular traps (NETs) in formaldehyde-fixed and paraffin-embedded feline cardiogenic arterial thrombi using heat-induced antigen retrieval and a double immunolabeling protocol.
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Duler, L., Nguyen, N., Ontiveros, E., Li, R. H. L. Identification of Neutrophil Extracellular Traps in Paraffin-Embedded Feline Arterial Thrombi using Immunofluorescence Microscopy. J. Vis. Exp. (157), e60834, doi:10.3791/60834 (2020).
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