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Immunologie et infection
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Identification of Neutrophil Extracellular Traps in Paraffin-Embedded Feline Arterial Thrombi using Immunofluorescence Microscopy
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Immunologie et infection
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Journal JoVE Immunologie et infection
Identification of Neutrophil Extracellular Traps in Paraffin-Embedded Feline Arterial Thrombi using Immunofluorescence Microscopy

Identification of Neutrophil Extracellular Traps in Paraffin-Embedded Feline Arterial Thrombi using Immunofluorescence Microscopy

DOI:
10.3791/60834-v

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08:48 min

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March 29, 2020

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, , ,
1Veterinary Medical Teaching Hospital, School of Veterinary Medicine,University of California, Davis, 2Department of Veterinary Surgical and Radiological Sciences, School of Veterinary Medicine,University of California, Davis, 3Department of Veterinary Medicine and Epidemiology, School of Veterinary Medicine,University of California, Davis

Chapitres

  • 00:04Introduction
  • 01:15Deparaffinization, Rehydration, and Heat-Induced Antigen Retrieval
  • 02:29Immunolabeling and Autofluorescence Quenching
  • 05:13Neutrophil Extracellular Trap Identification
  • 06:08Results: Phase Contrast and Immunofluorescence Images
  • 07:20Conclusion

Summary

Traduction automatique

Traduction automatique

We describe a method to identify neutrophil extracellular traps (NETs) in formaldehyde-fixed and paraffin-embedded feline cardiogenic arterial thrombi using heat-induced antigen retrieval and a double immunolabeling protocol.

Tags

Neutrophil Extracellular TrapsFeline Arterial ThrombiImmunofluorescence MicroscopyAntigen RetrievalCitrullinated Histone H3ThrombosisVeterinary Species

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