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Agrobacterium-Mediated Immature Embryo Transformation of Recalcitrant Maize Inbred Lines Using Morphogenic Genes
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Agrobacterium-Mediated Immature Embryo Transformation of Recalcitrant Maize Inbred Lines Using Morphogenic Genes

Agrobacterium-Mediated Immature Embryo Transformation of Recalcitrant Maize Inbred Lines Using Morphogenic Genes

21,252 Views

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10:28 min

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February 14, 2020

DOI:

10.3791/60782-v

DOI:
10.3791/60782-v

•

10:28 min
February 14, 2020

•

48 Views
, , , , , ,
1Department of Agronomy,Iowa State University, 2Department of Applied Science and Technology,Corteva Agriscience, 3Crop Bioengineering Center,Iowa State University, 4Interdepartmental Plant Biology Major,Iowa State University, 5Interdepartmental Genetics and Genomics Major,Iowa State University

Summary

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Plant morphogenic genes can be used to improve genetic transformation of recalcitrant genotypes. Described here is an Agrobacterium-mediated genetic transformation (QuickCorn) protocol for three important public maize inbred lines.

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Agrobacterium-mediatedImmature EmbryoTransformationRecalcitrantMaizeInbred LinesMorphogenic GenesBABY BOOMWUSCHELQuickCornCallus InductionT-DNACre/loxPHeat-induced RecombinationEmbryogenic GrowthPollenPollinationImmature Embryo SizeEmbryo Extraction

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Masters, A., Kang, M., McCaw, M., Zobrist, J. D., Gordon-Kamm, W., Jones, T., Wang, K. Agrobacterium-Mediated Immature Embryo Transformation of Recalcitrant Maize Inbred Lines Using Morphogenic Genes. J. Vis. Exp. (156), e60782, doi:10.3791/60782 (2020).

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