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Agrobacterium-Mediated Immature Embryo Transformation of Recalcitrant Maize Inbred Lines Using Morphogenic Genes
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Biologie
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JoVE Journal Biologie
Agrobacterium-Mediated Immature Embryo Transformation of Recalcitrant Maize Inbred Lines Using Morphogenic Genes

Agrobacterium-Mediated Immature Embryo Transformation of Recalcitrant Maize Inbred Lines Using Morphogenic Genes

21,263 Views

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10:28 min

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February 14, 2020

DOI:

10.3791/60782-v

DOI:
10.3791/60782-v

•

10:28 min
February 14, 2020

•

48 Views
, , , , , ,
1Department of Agronomy,Iowa State University, 2Department of Applied Science and Technology,Corteva Agriscience, 3Crop Bioengineering Center,Iowa State University, 4Interdepartmental Plant Biology Major,Iowa State University, 5Interdepartmental Genetics and Genomics Major,Iowa State University

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Plant morphogenic genes can be used to improve genetic transformation of recalcitrant genotypes. Described here is an Agrobacterium-mediated genetic transformation (QuickCorn) protocol for three important public maize inbred lines.

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Agrobacterium-mediatedImmature EmbryoTransformationRecalcitrantMaizeInbred LinesMorphogenic GenesBABY BOOMWUSCHELQuickCornCallus InductionT-DNACre/loxPHeat-induced RecombinationEmbryogenic GrowthPollenPollinationImmature Embryo SizeEmbryo Extraction

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Masters, A., Kang, M., McCaw, M., Zobrist, J. D., Gordon-Kamm, W., Jones, T., Wang, K. Agrobacterium-Mediated Immature Embryo Transformation of Recalcitrant Maize Inbred Lines Using Morphogenic Genes. J. Vis. Exp. (156), e60782, doi:10.3791/60782 (2020).

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