<em>Agrobacterium</em>-Mediated Immature Embryo Transformation of Recalcitrant Maize Inbred Lines Using Morphogenic Genes

Alicia Masters; Minjeong Kang; Morgan McCaw; Jacob D. Zobrist; William Gordon-Kamm; Todd Jones; Kan Wang

doi:10.3791/60782

Journal

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Biologie

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Agrobacterium-Mediated Immature Embryo Transformation of Recalcitrant Maize Inbred Lines Using Morphogenic Genes

Journal JoVE
Biologie

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Journal JoVE Biologie

Agrobacterium-Mediated Immature Embryo Transformation of Recalcitrant Maize Inbred Lines Using Morphogenic Genes

Agrobacterium-Mediated Immature Embryo Transformation of Recalcitrant Maize Inbred Lines Using Morphogenic Genes

21,263 Views

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10:28 min

•

February 14, 2020

DOI:

10.3791/60782-v

DOI:

10.3791/60782-v

•

10:28 min

February 14, 2020

•

48 Views

Alicia Masters^1,2, Minjeong Kang^1,3,4, Morgan McCaw^1,3, Jacob D. Zobrist^1,3,5, William Gordon-Kamm², Todd Jones², Kan Wang^1,3

¹Department of Agronomy,Iowa State University, ²Department of Applied Science and Technology,Corteva Agriscience, ³Crop Bioengineering Center,Iowa State University, ⁴Interdepartmental Plant Biology Major,Iowa State University, ⁵Interdepartmental Genetics and Genomics Major,Iowa State University

Summary

Plant morphogenic genes can be used to improve genetic transformation of recalcitrant genotypes. Described here is an Agrobacterium-mediated genetic transformation (QuickCorn) protocol for three important public maize inbred lines.

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Agrobacterium-mediated Immature Embryo Transformation Recalcitrant Maize Inbred Lines Morphogenic Genes BABY BOOM WUSCHEL QuickCorn Callus Induction T-DNA Cre/loxP Heat-induced Recombination Embryogenic Growth Pollen Pollination Immature Embryo Size Embryo Extraction

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Agrobacterium-Mediated Immature Embryo Transformation of Recalcitrant Maize Inbred Lines Using Morphogenic Genes

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