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Localization and Quantification of Begomoviruses in Whitefly Tissues by Immunofluorescence and Quantitative PCR
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Chapters
- 00:05Introduction
- 00:43Female Whitefly Midgut, Primary Salivary Gland (PSG), Ovary, and Hemolymph Dissection
- 02:03Tomato Yellow Leaf Curl Virus (TYLCV) Localization
- 03:40TYLCV Quantification
- 05:04Results: Representative TYLCV Detection and Quantification
- 05:46Conclusion
We describe an immunofluorescence and quantitative PCR method for the localization and quantification of begomoviruses in insect tissues. The immunofluorescence protocol can be used to colocalize viral and vector proteins. The quantitative PCR protocol can be extended to quantify viruses in whole whitefly bodies and virus-infected plants.
