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Immunology and Infection

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JoVE Journal Immunology and Infection
Localization and Quantification of Begomoviruses in Whitefly Tissues by Immunofluorescence and Quantitative PCR
 

Localization and Quantification of Begomoviruses in Whitefly Tissues by Immunofluorescence and Quantitative PCR

Article DOI: 10.3791/60731-v • 06:25 min • February 8th, 2020
February 8th, 2020 •
1, 1, 1, 1, 1, 1
1Ministry of Agriculture Key Laboratory of Molecular Biology of Crop Pathogen and Insects, Institute of Insect Sciences, Zhejiang University

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We describe an immunofluorescence and quantitative PCR method for the localization and quantification of begomoviruses in insect tissues. The immunofluorescence protocol can be used to colocalize viral and vector proteins. The quantitative PCR protocol can be extended to quantify viruses in whole whitefly bodies and virus-infected plants.

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Localization Quantification Begomoviruses Whitefly Tissues Immunofluorescence Quantitative PCR Virus-whitefly Interactions Control Strategies Technique Advantages Whitefly Proteins Virus Quantification Experimental Time Point Virus Acquisition Ice Anesthesia Female Whiteflies Microscope Slide Fine Acupuncture Needle Abdomen Opening Midgut Extraction Collection Dish PSGs (Prothoracic Salivary Glands) Thorax Splitting Needle Fixation Salivary Gland Separation Ovary Collection Hemolymph Sample Collection
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