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Localization and Quantification of Begomoviruses in Whitefly Tissues by Immunofluorescence and Quantitative PCR
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Localization and Quantification of Begomoviruses in Whitefly Tissues by Immunofluorescence and Quantitative PCR
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February 08, 2020
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We describe an immunofluorescence and quantitative PCR method for the localization and quantification of begomoviruses in insect tissues. The immunofluorescence protocol can be used to colocalize viral and vector proteins. The quantitative PCR protocol can be extended to quantify viruses in whole whitefly bodies and virus-infected plants.
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Ban, F., Yin, T., Guo, Q., Pan, L., Liu, Y., Wang, X. Localization and Quantification of Begomoviruses in Whitefly Tissues by Immunofluorescence and Quantitative PCR. J. Vis. Exp. (156), e60731, doi:10.3791/60731 (2020).
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