This content is Free Access.
Imágenes fluorescentes de calcio y posterior hibridación in situ para la caracterización de precursores neuronales en Xenopus laevis
7,567 Views
•
00:09 min
•
February 18, 2020
DOI:
1Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School,Harvard University, 2Department of Biology,College of William and Mary, 3Harvard Medical School,Harvard University, 4Department of Bioengineering,California Institute of Technology, 5Department of Computer Science,College of William and Mary
Summary
Automatically generated
Presentamos un protocolo de dos partes que combina imágenes fluorescentes de calcio con hibridación in situ, permitiendo al experimentador correlacionar patrones de actividad de calcio con perfiles de expresión génica a nivel de una sola célula.
Read Article
Cite this Article
Ablondi, E. F., Paudel, S., Sehdev, M., Marken, J. P., Halleran, A. D., Rahman, A., Kemper, P., Saha, M. S. Fluorescent Calcium Imaging and Subsequent In Situ Hybridization for Neuronal Precursor Characterization in Xenopus laevis. J. Vis. Exp. (156), e60726, doi:10.3791/60726 (2020).
Copy