Fluorescent Calcium Imaging and Subsequent In Situ Hybridization for Neuronal Precursor Characterization in <em>Xenopus laevis</em>

Eileen  F. Ablondi; Sudip Paudel; Morgan Sehdev; John  P. Marken; Andrew  D. Halleran; Atiqur Rahman; Peter Kemper; Margaret S. Saha

doi:10.3791/60726

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Fluorescent Calcium Imaging and Subsequent In Situ Hybridization for Neuronal Precursor Characterization in Xenopus laevis

JoVE Journal
発生生物学

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JoVE Journal 発生生物学

Fluorescent Calcium Imaging and Subsequent In Situ Hybridization for Neuronal Precursor Characterization in Xenopus laevis

Fluorescent Calcium Imaging and Subsequent In Situ Hybridization for Neuronal Precursor Characterization in Xenopus laevis

7,610 Views

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00:09 min

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February 18, 2020

DOI:

10.3791/60726-v

DOI:

10.3791/60726-v

•

00:09 min

February 18, 2020

•

3 Views

Eileen F. Ablondi¹, Sudip Paudel², Morgan Sehdev³, John P. Marken⁴, Andrew D. Halleran⁴, Atiqur Rahman⁵, Peter Kemper⁵, Margaret S. Saha²

¹Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School,Harvard University, ²生物学科,College of William and Mary, ³Harvard Medical School,Harvard University, ⁴生物工学科,California Institute of Technology, ⁵Department of Computer Science,College of William and Mary

概要

We present a two-part protocol that combines fluorescent calcium imaging with in situ hybridization, allowing the experimenter to correlate patterns of calcium activity with gene expression profiles on a single-cell level.

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Fluorescent Calcium Imaging and Subsequent In Situ Hybridization for Neuronal Precursor Characterization in Xenopus laevis

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