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Fluorescent Calcium Imaging and Subsequent In Situ Hybridization for Neuronal Precursor Characterization in Xenopus laevis
JoVE Journal
発生生物学
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JoVE Journal 発生生物学
Fluorescent Calcium Imaging and Subsequent In Situ Hybridization for Neuronal Precursor Characterization in Xenopus laevis

Fluorescent Calcium Imaging and Subsequent In Situ Hybridization for Neuronal Precursor Characterization in Xenopus laevis

DOI:
10.3791/60726-v

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00:09 min

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February 18, 2020

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, , , , , , ,
1Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School,Harvard University, 2生物学科,College of William and Mary, 3Harvard Medical School,Harvard University, 4生物工学科,California Institute of Technology, 5Department of Computer Science,College of William and Mary

章

  • 00:04Introduction
  • 00:34Dissection and Imaging Plate Preparation
  • 01:28Embryo Dissection
  • 05:27Calcium Imaging
  • 07:19Results: Representative Fluorescent Calcium Imaging and Subsequent In Situ Hybridization
  • 08:41Conclusion

概要

自動翻訳

自動翻訳

We present a two-part protocol that combines fluorescent calcium imaging with in situ hybridization, allowing the experimenter to correlate patterns of calcium activity with gene expression profiles on a single-cell level.

タグ

Fluorescent Calcium ImagingIn Situ HybridizationNeuronal PrecursorXenopus LaevisSingle Cell AnalysisCalcium ActivityGene ExpressionDissectionCollagenase

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