This content is Free Access.
Fluorescent Calcium Imaging and Subsequent In Situ Hybridization for Neuronal Precursor Characterization in Xenopus laevis
7,610 Views
•
00:09 min
•
February 18, 2020
DOI:
1Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School,Harvard University, 2Department of Biology,College of William and Mary, 3Harvard Medical School,Harvard University, 4Department of Bioengineering,California Institute of Technology, 5Department of Computer Science,College of William and Mary
Summary
Automatically generated
We present a two-part protocol that combines fluorescent calcium imaging with in situ hybridization, allowing the experimenter to correlate patterns of calcium activity with gene expression profiles on a single-cell level.
Read Article
Cite this Article
Ablondi, E. F., Paudel, S., Sehdev, M., Marken, J. P., Halleran, A. D., Rahman, A., Kemper, P., Saha, M. S. Fluorescent Calcium Imaging and Subsequent In Situ Hybridization for Neuronal Precursor Characterization in Xenopus laevis. J. Vis. Exp. (156), e60726, doi:10.3791/60726 (2020).
Copy