Dergi
/
Gelişim Biyolojisi
/
Fluorescent Calcium Imaging and Subsequent In Situ Hybridization for Neuronal Precursor Characterization in Xenopus laevis
JoVE Journal
Gelişim Biyolojisi
This content is Free Access.
JoVE Journal Gelişim Biyolojisi
Fluorescent Calcium Imaging and Subsequent In Situ Hybridization for Neuronal Precursor Characterization in Xenopus laevis

Fluorescent Calcium Imaging and Subsequent In Situ Hybridization for Neuronal Precursor Characterization in Xenopus laevis

7,610 Views

•

00:09 min

•

February 18, 2020

DOI:

10.3791/60726-v

DOI:
10.3791/60726-v

•

00:09 min
February 18, 2020

•

3 Views
, , , , , , ,
1Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School,Harvard University, 2Biyoloji Bölümü,Leonard I. Zon, 3Harvard Tıp Fakültesi,Harvard University, 4Biyomühendislik Bölümü,California Institute of Technology, 5Department of Computer Science,Leonard I. Zon

Özet

Automatically generated

We present a two-part protocol that combines fluorescent calcium imaging with in situ hybridization, allowing the experimenter to correlate patterns of calcium activity with gene expression profiles on a single-cell level.

Explore More Videos

Fluorescent Calcium ImagingIn Situ HybridizationNeuronal PrecursorXenopus LaevisSingle Cell AnalysisCalcium ActivityGene ExpressionDissectionCollagenase

Read Article

Cite this Article

Ablondi, E. F., Paudel, S., Sehdev, M., Marken, J. P., Halleran, A. D., Rahman, A., Kemper, P., Saha, M. S. Fluorescent Calcium Imaging and Subsequent In Situ Hybridization for Neuronal Precursor Characterization in Xenopus laevis. J. Vis. Exp. (156), e60726, doi:10.3791/60726 (2020).

Download .ris file

Copy

Share Video

.

Copy