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Fluorescent Calcium Imaging and Subsequent In Situ Hybridization for Neuronal Precursor Characterization in Xenopus laevis
JoVE Journal
Gelişim Biyolojisi
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JoVE Journal Gelişim Biyolojisi
Fluorescent Calcium Imaging and Subsequent In Situ Hybridization for Neuronal Precursor Characterization in Xenopus laevis

Fluorescent Calcium Imaging and Subsequent In Situ Hybridization for Neuronal Precursor Characterization in Xenopus laevis

DOI:
10.3791/60726-v

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00:09 min

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February 18, 2020

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, , , , , , ,
1Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School,Harvard University, 2Biyoloji Bölümü,Leonard I. Zon, 3Harvard Tıp Fakültesi,Harvard University, 4Biyomühendislik Bölümü,California Institute of Technology, 5Department of Computer Science,Leonard I. Zon

Bölümler

  • 00:04Introduction
  • 00:34Dissection and Imaging Plate Preparation
  • 01:28Embryo Dissection
  • 05:27Calcium Imaging
  • 07:19Results: Representative Fluorescent Calcium Imaging and Subsequent In Situ Hybridization
  • 08:41Conclusion

Özet

Otomatik Çeviri

Otomatik Çeviri

We present a two-part protocol that combines fluorescent calcium imaging with in situ hybridization, allowing the experimenter to correlate patterns of calcium activity with gene expression profiles on a single-cell level.

Etiketler

Fluorescent Calcium ImagingIn Situ HybridizationNeuronal PrecursorXenopus LaevisSingle Cell AnalysisCalcium ActivityGene ExpressionDissectionCollagenase

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