Journal
/
화학
/
Quantification of Metal Leaching in Immobilized Metal Affinity Chromatography
JoVE 신문
화학
JoVE 비디오를 활용하시려면 도서관을 통한 기관 구독이 필요합니다.  전체 비디오를 보시려면 로그인하거나 무료 트라이얼을 시작하세요.
JoVE 신문 화학
Quantification of Metal Leaching in Immobilized Metal Affinity Chromatography

Quantification of Metal Leaching in Immobilized Metal Affinity Chromatography

DOI:
10.3791/60690-v

•

05:35 min

•

January 17, 2020

•

, , ,
1Department of Chemistry and Biochemistry,James Madison University, 2Biophysics Graduate Program,Ohio State University

Chapters

  • 00:04Title
  • 00:55Assay Component Preparation
  • 01:36Sample Preparation, Measurement, and Metal Quantification
  • 03:24Results: Absorbance Spectra and Ni2+ Quantification
  • 04:42Conclusion

Summary

자동 번역

자동 번역

We present an assay for easy quantification of metals introduced to samples prepared using immobilized metal affinity chromatography. The method uses hydroxynaphthol blue as the colorimetric metal indicator and a UV-Vis spectrophotometer as the detector.

Tags

Metal LeachingImmobilized Metal Affinity ChromatographyUV-Vis SpectrophotometryHNB ReagentProtein QuantificationBuffer SolutionSpectral AnalysisSample PreparationMetal Concentration Determination

Article

Embed

플레이리스트에 추가

Usage Statistics

Related Videos

Synthesis and Characterization of Functionalized Metal-organic Frameworks

Radiolabeling and Quantification of Cellular Levels of Phosphoinositides by High Performance Liquid Chromatography-coupled Flow Scintillation

Synthesis of a Water-soluble Metal–Organic Complex Array

In Situ Detection and Single Cell Quantification of Metal Oxide Nanoparticles Using Nuclear Microprobe Analysis

Ligand-Mediated Nucleation and Growth of Palladium Metal Nanoparticles

Surface Functionalization of Metal-Organic Frameworks for Improved Moisture Resistance

Electrophoretic Crystallization of Ultrathin High-performance Metal-organic Framework Membranes

Molten-Salt Synthesis of Complex Metal Oxide Nanoparticles

Ion Mobility-Mass Spectrometry Techniques for Determining the Structure and Mechanisms of Metal Ion Recognition and Redox Activity of Metal Binding Oligopeptides

Localized Bathless Metal-Composite Plating via Electrostamping

Read Article