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Quantification of Metal Leaching in Immobilized Metal Affinity Chromatography
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JoVE Journal Chemistry
Quantification of Metal Leaching in Immobilized Metal Affinity Chromatography

Quantification of Metal Leaching in Immobilized Metal Affinity Chromatography

English

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7,044 Views

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05:35 min

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January 17, 2020

DOI:

10.3791/60690-v

DOI:
10.3791/60690-v

•

05:35 min
January 17, 2020

•

10 Views
, , ,
1Department of Chemistry and Biochemistry,James Madison University, 2Biophysics Graduate Program,Ohio State University

Summary

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We present an assay for easy quantification of metals introduced to samples prepared using immobilized metal affinity chromatography. The method uses hydroxynaphthol blue as the colorimetric metal indicator and a UV-Vis spectrophotometer as the detector.

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Swaim, C. M., Brittain, T. J., Marzolf, D. R., Kokhan, O. Quantification of Metal Leaching in Immobilized Metal Affinity Chromatography. J. Vis. Exp. (155), e60690, doi:10.3791/60690 (2020).

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