需要订阅 JoVE 才能查看此. 登录或开始免费试用。
Quantification of Metal Leaching in Immobilized Metal Affinity Chromatography
7,044 Views
•
05:35 min
•
January 17, 2020
DOI:
Summary
Automatically generated
We present an assay for easy quantification of metals introduced to samples prepared using immobilized metal affinity chromatography. The method uses hydroxynaphthol blue as the colorimetric metal indicator and a UV-Vis spectrophotometer as the detector.
Read Article
Cite this Article
Swaim, C. M., Brittain, T. J., Marzolf, D. R., Kokhan, O. Quantification of Metal Leaching in Immobilized Metal Affinity Chromatography. J. Vis. Exp. (155), e60690, doi:10.3791/60690 (2020).
Copy