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ヒト幹細胞由来内皮細胞およびGABAergicニューロンの移動、化学誘引、共培養アッセイ
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Migration, Chemo-Attraction, and Co-Culture Assays for Human Stem Cell-Derived Endothelial Cells and GABAergic Neurons
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ヒト幹細胞由来内皮細胞およびGABAergicニューロンの移動、化学誘引、共培養アッセイ

6,857 Views

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10:09 min

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January 23, 2020

DOI:

10.3791/60295-v

DOI:
10.3791/60295-v

•

10:09 min
January 23, 2020

•

16 Views
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1Department of Psychiatry,Harvard Medical School, 2Angiogenesis and Brain Development Laboratory, Division of Basic Neuroscience,McLean Hospital

Summary

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我々は、3つの単純なインビトロアッセイ-長距離移動アッセイ、共培養移行アッセイ、および化学誘引アッセイ-それは、ヒト幹細胞由来の心室内皮細胞とその機能を一括して評価するGABAergic インターニューロンとの相互作用.

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Keyword Extraction:MigrationChemo-attractionCo-cultureHuman Stem Cell-derived Endothelial CellsGABAergic NeuronsPeriventricular Endothelial CellsBrain DisordersAutismEpilepsySchizophreniaDepressionCell MigrationCell DissociationCell CountingTrypan Blue ExclusionNeuronal MediumEndothelial MediumCell CultureCell Assays

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Datta, D., Vasudevan, A. Migration, Chemo-Attraction, and Co-Culture Assays for Human Stem Cell-Derived Endothelial Cells and GABAergic Neurons. J. Vis. Exp. (155), e60295, doi:10.3791/60295 (2020).

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