Dergi
/
Nörobilim
/
Migration, Chemo-Attraction, and Co-Culture Assays for Human Stem Cell-Derived Endothelial Cells and GABAergic Neurons
JoVE Journal
Nörobilim
Bu içeriği görüntülemek için JoVE aboneliği gereklidir.  Oturum açın veya ücretsiz deneme sürümünü başlatın.
JoVE Journal Nörobilim
Migration, Chemo-Attraction, and Co-Culture Assays for Human Stem Cell-Derived Endothelial Cells and GABAergic Neurons

Migration, Chemo-Attraction, and Co-Culture Assays for Human Stem Cell-Derived Endothelial Cells and GABAergic Neurons

DOI:
10.3791/60295-v

•

10:09 min

•

January 23, 2020

•

,
1Psikiyatri Bölümü,Harvard Tıp Fakültesi, 2Angiogenesis and Brain Development Laboratory, Division of Basic Neuroscience,McLean Hospital

Bölümler

  • 00:05Başlık
  • 01:17Preparation of Human Cells for Assay
  • 03:36Preparation of One-Well Culture Inserts
  • 04:12Long-Distance Migration Assay
  • 05:48Co-culture Migration and Chemo-Attraction Assays
  • 07:28Results: Migration and Chemo-Attraction Assays
  • 08:38Conclusion

Özet

Otomatik Çeviri

Otomatik Çeviri

We present three simple in vitro assays-the long-distance migration assay, the co-culture migration assay, and chemo-attraction assay-that collectively evaluate the functions of human stem cell derived periventricular endothelial cells and their interaction with GABAergic interneurons.

Etiketler

Keyword Extraction:MigrationChemo-attractionCo-cultureHuman Stem Cell-derived Endothelial CellsGABAergic NeuronsPeriventricular Endothelial CellsBrain DisordersAutismEpilepsySchizophreniaDepressionCell MigrationCell DissociationCell CountingTrypan Blue ExclusionNeuronal MediumEndothelial MediumCell CultureCell Assays

Article

Embed

ÇALMA LİSTESİNE EKLE

Usage Statistics

İlgili Videolar

Bilaminar Co-culture of Primary Rat Cortical Neurons and Glia

Inhibitory Synapse Formation in a Co-culture Model Incorporating GABAergic Medium Spiny Neurons and HEK293 Cells Stably Expressing GABAA Receptors

Dorsal Root Ganglia Neurons and Differentiated Adipose-derived Stem Cells: An In Vitro Co-culture Model to Study Peripheral Nerve Regeneration

Enumeration of Neural Stem Cells Using Clonal Assays

Modelling Zika Virus Infection of the Developing Human Brain In Vitro Using Stem Cell Derived Cerebral Organoids

Method for High Speed Stretch Injury of Human Induced Pluripotent Stem Cell-derived Neurons in a 96-well Format

Compartmentalization of Human Stem Cell-Derived Neurons within Pre-Assembled Plastic Microfluidic Chips

Post-differentiation Replating of Human Pluripotent Stem Cell-derived Neurons for High-content Screening of Neuritogenesis and Synapse Maturation

Analyzing Mitochondrial Transport and Morphology in Human Induced Pluripotent Stem Cell-Derived Neurons in Hereditary Spastic Paraplegia

Derivation, Expansion, Cryopreservation and Characterization of Brain Microvascular Endothelial Cells from Human Induced Pluripotent Stem Cells

Read Article