/
/
Migration, Chemo-Attraction, and Co-Culture Assays for Human Stem Cell-Derived Endothelial Cells and GABAergic Neurons
Se requiere una suscripción a JoVE para ver este contenido. Inicie sesión o comience su prueba gratuita.
Migration, Chemo-Attraction, and Co-Culture Assays for Human Stem Cell-Derived Endothelial Cells and GABAergic Neurons
6,861 Views
•
10:09 min
•
January 23, 2020
DOI:
Summary
Automatically generated
We present three simple in vitro assays-the long-distance migration assay, the co-culture migration assay, and chemo-attraction assay-that collectively evaluate the functions of human stem cell derived periventricular endothelial cells and their interaction with GABAergic interneurons.
Explore More Videos
Keyword Extraction:MigrationChemo-attractionCo-cultureHuman Stem Cell-derived Endothelial CellsGABAergic NeuronsPeriventricular Endothelial CellsBrain DisordersAutismEpilepsySchizophreniaDepressionCell MigrationCell DissociationCell CountingTrypan Blue ExclusionNeuronal MediumEndothelial MediumCell CultureCell Assays
Read Article
Cite this Article
Datta, D., Vasudevan, A. Migration, Chemo-Attraction, and Co-Culture Assays for Human Stem Cell-Derived Endothelial Cells and GABAergic Neurons. J. Vis. Exp. (155), e60295, doi:10.3791/60295 (2020).
Copy