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Migration, Chemo-Attraction, and Co-Culture Assays for Human Stem Cell-Derived Endothelial Cells and GABAergic Neurons
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Migration, Chemo-Attraction, and Co-Culture Assays for Human Stem Cell-Derived Endothelial Cells and GABAergic Neurons
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January 23, 2020
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We present three simple in vitro assays-the long-distance migration assay, the co-culture migration assay, and chemo-attraction assay-that collectively evaluate the functions of human stem cell derived periventricular endothelial cells and their interaction with GABAergic interneurons.
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Keyword Extraction:MigrationChemo-attractionCo-cultureHuman Stem Cell-derived Endothelial CellsGABAergic NeuronsPeriventricular Endothelial CellsBrain DisordersAutismEpilepsySchizophreniaDepressionCell MigrationCell DissociationCell CountingTrypan Blue ExclusionNeuronal MediumEndothelial MediumCell CultureCell Assays
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Datta, D., Vasudevan, A. Migration, Chemo-Attraction, and Co-Culture Assays for Human Stem Cell-Derived Endothelial Cells and GABAergic Neurons. J. Vis. Exp. (155), e60295, doi:10.3791/60295 (2020).
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