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Visualizing and Analyzing Intracellular Transport of Organelles and Other Cargos in Astrocytes
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August 28, 2019
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Here we describe an in vitro live-imaging method to visualize intracellular transport of organelles and trafficking of plasma membrane proteins in murine astrocytes. This protocol also presents an image analysis methodology to determine cargo transport itineraries and kinetics.
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Creighton, B. A., Ruffins, T. W., Lorenzo, D. N. Visualizing and Analyzing Intracellular Transport of Organelles and Other Cargos in Astrocytes. J. Vis. Exp. (150), e60230, doi:10.3791/60230 (2019).
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