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Visualizing and Analyzing Intracellular Transport of Organelles and Other Cargos in Astrocytes
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JoVE Journal Nörobilim
Visualizing and Analyzing Intracellular Transport of Organelles and Other Cargos in Astrocytes

Visualizing and Analyzing Intracellular Transport of Organelles and Other Cargos in Astrocytes

7,568 Views

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00:07 min

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August 28, 2019

DOI:

10.3791/60230-v

DOI:
10.3791/60230-v

•

00:07 min
August 28, 2019

•

2 Views
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1Department of Cell Biology and Physiology,University of North Carolina at Chapel Hill

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Here we describe an in vitro live-imaging method to visualize intracellular transport of organelles and trafficking of plasma membrane proteins in murine astrocytes. This protocol also presents an image analysis methodology to determine cargo transport itineraries and kinetics.

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Creighton, B. A., Ruffins, T. W., Lorenzo, D. N. Visualizing and Analyzing Intracellular Transport of Organelles and Other Cargos in Astrocytes. J. Vis. Exp. (150), e60230, doi:10.3791/60230 (2019).

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