Rivista
/
Neuroscienze
/
Visualizing and Analyzing Intracellular Transport of Organelles and Other Cargos in Astrocytes
JoVE Journal
Neuroscienze
È necessario avere un abbonamento a JoVE per visualizzare questo.  Accedi o inizia la tua prova gratuita.
JoVE Journal Neuroscienze
Visualizing and Analyzing Intracellular Transport of Organelles and Other Cargos in Astrocytes

Visualizing and Analyzing Intracellular Transport of Organelles and Other Cargos in Astrocytes

DOI:
10.3791/60230-v

•

00:07 min

•

August 28, 2019

•

, ,
1Department of Cell Biology and Physiology,University of North Carolina at Chapel Hill

Capitoli

  • 00:04Titolo
  • 00:31Fluorescently-Tagged Plasmid Transfection and Fluorescent Probe Late Endosome/Lysosome Labeling
  • 02:13Time-Lapse Imaging
  • 03:03Image Analysis
  • 05:00Results: Representative Quantitative Analyses of Cargo Dynamics in Astrocytes
  • 06:38Conclusion

Summary

Traduzione automatica

Traduzione automatica

Here we describe an in vitro live-imaging method to visualize intracellular transport of organelles and trafficking of plasma membrane proteins in murine astrocytes. This protocol also presents an image analysis methodology to determine cargo transport itineraries and kinetics.

Tags

Intracellular TransportOrganelle MotilityAstrocytesLive ImagingFluorescent LabelingLipofectionTransfectionTime-lapse MicroscopyImage Analysis

Article

Embed

AGGIUNGI ALLA PLAYLIST

Usage Statistics

Video correlati

Fluorescence Recovery After Photobleaching (FRAP) of Fluorescence Tagged Proteins in Dendritic Spines of Cultured Hippocampal Neurons

Isolation and Culture of Mouse Cortical Astrocytes

Imaging Intracellular Ca2+ Signals in Striatal Astrocytes from Adult Mice Using Genetically-encoded Calcium Indicators

Protocol for Three-dimensional Confocal Morphometric Analysis of Astrocytes

Analyzing Dendritic Morphology in Columns and Layers

Visualization and Live Imaging of Oligodendrocyte Organelles in Organotypic Brain Slices Using Adeno-associated Virus and Confocal Microscopy

Analyzing the Size, Shape, and Directionality of Networks of Coupled Astrocytes

Analyzing Mitochondrial Transport and Morphology in Human Induced Pluripotent Stem Cell-Derived Neurons in Hereditary Spastic Paraplegia

Axonal Transport of Organelles in Motor Neuron Cultures using Microfluidic Chambers System

In Vitro Wedge Slice Preparation for Mimicking In Vivo Neuronal Circuit Connectivity

Read Article