Journal
/
/
Generatie chimeric Axolotls met Mutant Haploid Ledematen door embryonale grafting
JoVE 신문
유전학
JoVE 비디오를 활용하시려면 도서관을 통한 기관 구독이 필요합니다.  전체 비디오를 보시려면 로그인하거나 무료 트라이얼을 시작하세요.
JoVE 신문 유전학
Generation of Chimeric Axolotls with Mutant Haploid Limbs Through Embryonic Grafting

Generatie chimeric Axolotls met Mutant Haploid Ledematen door embryonale grafting

DOI:

07:17 min

January 29, 2020

, ,

Chapters

  • 00:05Title
  • 00:48Female Gamete Donor Preparation
  • 01:36Male Gamete Collection and Preparation
  • 02:51Female Gamete Collection
  • 03:48Haploid-Diploid Chimera Generation
  • 05:07Results: Representative Haploid and Diploid Limb Development
  • 06:30Conclusion

Summary

자동 번역

Dit doel van dit protocol is het produceren van chimerische axolotls met haploïde voorpoten afgeleid van Cas9-mutagenized donorweefsel met behulp van embryonale weefsel enten technieken.

Related Videos

Read Article