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Generation of Chimeric Axolotls with Mutant Haploid Limbs Through Embryonic Grafting
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Genetics
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JoVE Journal Genetics
Generation of Chimeric Axolotls with Mutant Haploid Limbs Through Embryonic Grafting

Generation of Chimeric Axolotls with Mutant Haploid Limbs Through Embryonic Grafting

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7,051 Views

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07:17 min

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January 29, 2020

DOI:

10.3791/60156-v

DOI:
10.3791/60156-v

•

07:17 min
January 29, 2020

•

12 Views
, ,
1Department of Molecular, Cellular, and Developmental Biology,Yale University

Summary

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This goal of this protocol is to produce chimeric axolotls with haploid forelimbs derived from Cas9-mutagenized donor tissue using embryonic tissue grafting techniques.

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Sanor, L. D., Flowers, G. P., Crews, C. M. Generation of Chimeric Axolotls with Mutant Haploid Limbs Through Embryonic Grafting. J. Vis. Exp. (155), e60156, doi:10.3791/60156 (2020).

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