This goal of this protocol is to produce chimeric axolotls with haploid forelimbs derived from Cas9-mutagenized donor tissue using embryonic tissue grafting techniques.
Sanor, L. D., Flowers, G. P., Crews, C. M. Generation of Chimeric Axolotls with Mutant Haploid Limbs Through Embryonic Grafting. J. Vis. Exp. (155), e60156, doi:10.3791/60156 (2020).