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Generation of Chimeric Axolotls with Mutant Haploid Limbs Through Embryonic Grafting
JoVE Journal
Genetics
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JoVE Journal Genetics
Generation of Chimeric Axolotls with Mutant Haploid Limbs Through Embryonic Grafting

Generation of Chimeric Axolotls with Mutant Haploid Limbs Through Embryonic Grafting

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07:17 min

January 29, 2020

DOI:

07:17 min
January 29, 2020

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Summary

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This goal of this protocol is to produce chimeric axolotls with haploid forelimbs derived from Cas9-mutagenized donor tissue using embryonic tissue grafting techniques.

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