需要订阅 JoVE 才能查看此. 登录或开始免费试用。
Generation of Chimeric Axolotls with Mutant Haploid Limbs Through Embryonic Grafting
7,044 Views
•
07:17 min
•
January 29, 2020
DOI:
Summary
Automatically generated
This goal of this protocol is to produce chimeric axolotls with haploid forelimbs derived from Cas9-mutagenized donor tissue using embryonic tissue grafting techniques.
Read Article
Cite this Article
Sanor, L. D., Flowers, G. P., Crews, C. M. Generation of Chimeric Axolotls with Mutant Haploid Limbs Through Embryonic Grafting. J. Vis. Exp. (155), e60156, doi:10.3791/60156 (2020).
Copy