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Generation of Chimeric Axolotls with Mutant Haploid Limbs Through Embryonic Grafting
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January 29, 2020
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This goal of this protocol is to produce chimeric axolotls with haploid forelimbs derived from Cas9-mutagenized donor tissue using embryonic tissue grafting techniques.
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Sanor, L. D., Flowers, G. P., Crews, C. M. Generation of Chimeric Axolotls with Mutant Haploid Limbs Through Embryonic Grafting. J. Vis. Exp. (155), e60156, doi:10.3791/60156 (2020).
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