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从人类和小鼠心肌标本中分离巨噬细胞子集和细胞位细胞
JoVE 신문
면역학 및 감염병학
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JoVE 신문 면역학 및 감염병학
Isolation of Macrophage Subsets and Stromal Cells from Human and Mouse Myocardial Specimens
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从人类和小鼠心肌标本中分离巨噬细胞子集和细胞位细胞

DOI:
10.3791/60015-v

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07:25 min

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December 17, 2019

•

,
1Department of Medicine,Washington University School of Medicine, 2Department of Immunology and Pathology,Washington University School of Medicine, 3Department of Developmental Biology,Washington University School of Medicine

Chapters

  • 00:05Title
  • 01:14Preparation of Human Cardiac Tissue Specimens
  • 01:41Preparation of the Mouse Heart
  • 02:11Preparation of Single Cell Suspension
  • 04:50Antibody Staining
  • 05:33Results: Flow Cytometry Gating Scheme
  • 06:22Conclusion

Summary

자동 번역

자동 번역

这里介绍的一个协议，通过酶消化制备单个细胞悬浮液，从人类和小鼠心肌中分离出巨噬细胞和其他非免疫细胞的各种子集。提出了基于流细胞学的识别与分离巨噬细胞表征的门控方案。

Tags

Macrophage SubsetsStromal CellsCardiac InflammationCardiac RepairImmune Cell DiversitySingle Cell SuspensionViable Immune CellsNonimmune Cell PopulationsMurine HeartCollagenase IDNase IHyaluronidaseDMEMDigestion

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