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Isolation of Macrophage Subsets and Stromal Cells from Human and Mouse Myocardial Specimens
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Isolation of Macrophage Subsets and Stromal Cells from Human and Mouse Myocardial Specimens
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December 17, 2019
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Presented here is a protocol to isolate various subsets of macrophages and other non-immune cells from human and mouse myocardium by preparing a single cell suspension through enzymatic digestion. Gating schemes for flow cytometry based identification and characterization of isolated macrophages are also presented.
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Bajpai, G., Lavine, K. J. Isolation of Macrophage Subsets and Stromal Cells from Human and Mouse Myocardial Specimens. J. Vis. Exp. (154), e60015, doi:10.3791/60015 (2019).
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