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Isolation of Macrophage Subsets and Stromal Cells from Human and Mouse Myocardial Specimens
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Inmunología y contagio
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JoVE Revista Inmunología y contagio
Isolation of Macrophage Subsets and Stromal Cells from Human and Mouse Myocardial Specimens

Isolation of Macrophage Subsets and Stromal Cells from Human and Mouse Myocardial Specimens

7,366 Views

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07:25 min

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December 17, 2019

DOI:

10.3791/60015-v

DOI:
10.3791/60015-v

•

07:25 min
December 17, 2019

•

9 Views
,
1Department of Medicine,Washington University School of Medicine, 2Department of Immunology and Pathology,Washington University School of Medicine, 3Department of Developmental Biology,Washington University School of Medicine

Summary

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Presented here is a protocol to isolate various subsets of macrophages and other non-immune cells from human and mouse myocardium by preparing a single cell suspension through enzymatic digestion. Gating schemes for flow cytometry based identification and characterization of isolated macrophages are also presented.

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Bajpai, G., Lavine, K. J. Isolation of Macrophage Subsets and Stromal Cells from Human and Mouse Myocardial Specimens. J. Vis. Exp. (154), e60015, doi:10.3791/60015 (2019).

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