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Isolation of Macrophage Subsets and Stromal Cells from Human and Mouse Myocardial Specimens
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Immunology and Infection
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JoVE Journal Immunology and Infection
Isolation of Macrophage Subsets and Stromal Cells from Human and Mouse Myocardial Specimens

Isolation of Macrophage Subsets and Stromal Cells from Human and Mouse Myocardial Specimens

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07:25 min

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December 17, 2019

DOI:

10.3791/60015-v

DOI:
10.3791/60015-v

•

07:25 min
December 17, 2019

•

1 Views
,
1Department of Medicine,Washington University School of Medicine, 2Department of Immunology and Pathology,Washington University School of Medicine, 3Department of Developmental Biology,Washington University School of Medicine

Tags

Macrophage SubsetsStromal CellsCardiac InflammationCardiac RepairImmune Cell DiversitySingle Cell SuspensionViable Immune CellsNonimmune Cell PopulationsMurine HeartCollagenase IDNase IHyaluronidaseDMEMDigestion

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Bajpai, G., Lavine, K. J. Isolation of Macrophage Subsets and Stromal Cells from Human and Mouse Myocardial Specimens. J. Vis. Exp. (154), e60015, doi:10.3791/60015 (2019).

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