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Presynapse Formation Assay Using Presynapse Organizer Beads and “Neuron Ball” Culture
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Presynapse Formation Assay Using Presynapse Organizer Beads and “Neuron Ball” Culture

Presynapse Formation Assay Using Presynapse Organizer Beads and “Neuron Ball” Culture

8,056 Views

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10:17 min

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August 02, 2019

DOI:

10.3791/59893-v

DOI:
10.3791/59893-v

•

10:17 min
August 02, 2019

•

4 Views
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1Functional Structure Biology Laboratory, Department of Medical Life Science,Yokohama City University Graduate School of Medical Life Science

Summary

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Presynapse formation is a dynamic process including accumulation of synaptic proteins in proper order. In this method, presynapse formation is triggered by beads conjugated with a presynapse organizer protein on axonal sheets of “neuron ball” culture, so that accumulation of synaptic proteins is easy to be analyzed during presynapse formation.

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Parvin, S., Takeda, R., Sasaki, Y. Presynapse Formation Assay Using Presynapse Organizer Beads and “Neuron Ball” Culture. J. Vis. Exp. (150), e59893, doi:10.3791/59893 (2019).

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