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Presynapse Formation Assay Using Presynapse Organizer Beads and “Neuron Ball” Culture
JoVE Revista
Neurociencias
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JoVE Revista Neurociencias
Presynapse Formation Assay Using Presynapse Organizer Beads and “Neuron Ball” Culture

Presynapse Formation Assay Using Presynapse Organizer Beads and “Neuron Ball” Culture

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10:17 min

August 02, 2019

DOI:

10:17 min
August 02, 2019

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Summary

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Presynapse formation is a dynamic process including accumulation of synaptic proteins in proper order. In this method, presynapse formation is triggered by beads conjugated with a presynapse organizer protein on axonal sheets of “neuron ball” culture, so that accumulation of synaptic proteins is easy to be analyzed during presynapse formation.

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