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Presynapse Formation Assay Using Presynapse Organizer Beads and “Neuron Ball” Culture
章
- 00:04標題
- 00:55Preparation of Neuron Balls as Hanging Drop Culture
- 03:24Placing Neuron Balls on PLL-Coated Glass Coverslips and Culture Maintenance
- 04:29Applying LRRTM2 Beads on Neuron Ball Culture With or Without Cell Bodies
- 06:07Fluorescence Microscopy
- 07:19Results: Analysis of Accumulation of Presynaptic Proteins in LRRTM2-Induced Presynapses of Axons of Neuron Ball Culture
- 09:15Conclusion
Presynapse formation is a dynamic process including accumulation of synaptic proteins in proper order. In this method, presynapse formation is triggered by beads conjugated with a presynapse organizer protein on axonal sheets of “neuron ball” culture, so that accumulation of synaptic proteins is easy to be analyzed during presynapse formation.
