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Genetics

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JoVE Journal Genetics
Transcription Start Site Mapping Using Super-low Input Carrier-CAGE
 

Transcription Start Site Mapping Using Super-low Input Carrier-CAGE

Article DOI: 10.3791/59805-v • 06:59 min • June 26th, 2019
June 26th, 2019 •
1,2, 1,2, 1,2,3
1Institute of Clinical Sciences, Faculty of Medicine, Imperial College London, 2MRC London Institute of Medical Sciences, 3Sars International Centre for Marine Molecular Biology, University of Bergen

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요약
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Cap Analysis of Gene Expression (CAGE) is a method for genome-wide quantitative mapping of mRNA 5’ends to capture RNA polymerase II transcription start sites at a single-nucleotide resolution. This work describes a low-input (SLIC-CAGE) protocol for generation of high-quality libraries using nanogram-amounts of total RNA.

Tags

Transcription Start Site Mapping Super-low Input Carrier-CAGE Gene Regulation Core Promoter Enhancer Discovery Starting Material Unbiased Genome-wide Single Nucleotide Resolution Mapping RNA Polymerase II Transcriptional Start Sites Total RNA CAGE Disease-associated Transcription Start Sites Diagnostic Markers SLIC-CAGE Tissue Biopsies Promoter Analysis Frail Cell Types Early Embryonic Developmental Stages Embryonic Tissue Model Organisms PCR Mix Buffer DNTPs Forward Primer Template Plasmid Synthetic Carrier Gene Fusion Polymerase Thermocycling Conditions Reverse Transcription
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