This content is Free Access.
Transcription Start Site Mapping Using Super-low Input Carrier-CAGE
11,728 Views
•
06:59 min
•
June 26, 2019
DOI:
Summary
Automatically generated
Cap Analysis of Gene Expression (CAGE) is a method for genome-wide quantitative mapping of mRNA 5’ends to capture RNA polymerase II transcription start sites at a single-nucleotide resolution. This work describes a low-input (SLIC-CAGE) protocol for generation of high-quality libraries using nanogram-amounts of total RNA.
Read Article
Cite this Article
Cvetesic, N., Pahita, E., Lenhard, B. Transcription Start Site Mapping Using Super-low Input Carrier-CAGE. J. Vis. Exp. (148), e59805, doi:10.3791/59805 (2019).
Copy