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Transcription Start Site Mapping Using Super-low Input Carrier-CAGE
JoVE Journal
Genetica
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JoVE Journal Genetica
Transcription Start Site Mapping Using Super-low Input Carrier-CAGE

Transcription Start Site Mapping Using Super-low Input Carrier-CAGE

11,731 Views

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06:59 min

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June 26, 2019

DOI:

10.3791/59805-v

DOI:
10.3791/59805-v

•

06:59 min
June 26, 2019

•

3 Views
, ,
1Institute of Clinical Sciences, Faculty of Medicine,Imperial College London, 2MRC London Institute of Medical Sciences, 3Sars International Centre for Marine Molecular Biology,University of Bergen

Summary

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Cap Analysis of Gene Expression (CAGE) is a method for genome-wide quantitative mapping of mRNA 5’ends to capture RNA polymerase II transcription start sites at a single-nucleotide resolution. This work describes a low-input (SLIC-CAGE) protocol for generation of high-quality libraries using nanogram-amounts of total RNA.

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Cite this Article

Cvetesic, N., Pahita, E., Lenhard, B. Transcription Start Site Mapping Using Super-low Input Carrier-CAGE. J. Vis. Exp. (148), e59805, doi:10.3791/59805 (2019).

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