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In Vitro ELISA Test to Evaluate Rabies Vaccine Potency
Chapters
- 00:04Introduction
- 01:08Microplate Sensitization
- 02:07Microplate Passivation
- 02:33ELISA Assay
- 06:44Results: Analysis of the ELISA Test
- 08:00Conclusion
Here we describe an indirect ELISA sandwich immunocapture to determine the immunogenic glycoprotein contents in rabies vaccines. This test uses a neutralizing Monoclonal Antibody (mAb-D1) recognizing glycoprotein trimers. It is an alternative to the in vivo NIH test to follow the consistency of vaccine potency during production.
