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In Vitro ELISA Test to Evaluate Rabies Vaccine Potency
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In Vitro ELISA Test to Evaluate Rabies Vaccine Potency

In Vitro ELISA Test to Evaluate Rabies Vaccine Potency

11,938 Views

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09:04 min

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May 11, 2020

DOI:

10.3791/59641-v

DOI:
10.3791/59641-v

•

09:04 min
May 11, 2020

•

33 Views
,
1Unit Antiviral Strategies, OIE Reference Laboratories for Rift Valley Fever Virus & Crimean Congo Hemorrhagic Fever Virus,Institut Pasteur, 2Institut Pasteur de Guinée, Conakry

Summary

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Here we describe an indirect ELISA sandwich immunocapture to determine the immunogenic glycoprotein contents in rabies vaccines. This test uses a neutralizing Monoclonal Antibody (mAb-D1) recognizing glycoprotein trimers. It is an alternative to the in vivo NIH test to follow the consistency of vaccine potency during production.

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Cite this Article

Jallet, C., Tordo, N. In Vitro ELISA Test to Evaluate Rabies Vaccine Potency. J. Vis. Exp. (159), e59641, doi:10.3791/59641 (2020).

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