Zum Anzeigen dieser Inhalte ist ein JoVE-Abonnement erforderlich. Melden Sie sich an oder starten Sie Ihre kostenlose Testversion.
In Vitro ELISA Test to Evaluate Rabies Vaccine Potency
11,938 Views
•
09:04 min
•
May 11, 2020
DOI:
Summary
Automatically generated
Here we describe an indirect ELISA sandwich immunocapture to determine the immunogenic glycoprotein contents in rabies vaccines. This test uses a neutralizing Monoclonal Antibody (mAb-D1) recognizing glycoprotein trimers. It is an alternative to the in vivo NIH test to follow the consistency of vaccine potency during production.
Read Article
Cite this Article
Jallet, C., Tordo, N. In Vitro ELISA Test to Evaluate Rabies Vaccine Potency. J. Vis. Exp. (159), e59641, doi:10.3791/59641 (2020).
Copy