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使用声学纳米分配技术实现高通量DNA质粒多路复用和转染
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High-Throughput DNA Plasmid Multiplexing and Transfection Using Acoustic Nanodispensing Technology

使用声学纳米分配技术实现高通量DNA质粒多路复用和转染

DOI:

13:27 min

August 08, 2019

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Chapters

  • 00:04Title
  • 00:47Preparation of the Peristaltic Liquid Handler Programs
  • 01:53Experimental Design and Generation of the Picklists to Drive the Acoustic Droplet Ejection (ADE)-Based Dispenses
  • 03:34DNA Source Plate Preparation Using the 384-well Pipetting Guide Application and Survey Performance to Control the Manually Dispensed Volumes
  • 06:18Peristaltic Liquid Handler-Based 1 μL Diluent Dispensation in the Destination Plate
  • 07:03ADE-driven DNA Dispensing into the Destination Plate and ADE-driven Transfection Reagent Dispensing
  • 09:54Peristaltic Liquid Handler-Based Cell Dispensation
  • 10:51Results: Defining the Key Parameters to Reach Highly Efficient and High Thoughput Cells (Co-) Transfections
  • 12:26Conclusion

Summary

자동 번역

该协议使用声滴喷射技术描述了384孔板中哺乳动物细胞的高通量质粒转染。耗时、易出错的 DNA 点胶和复用,以及转染试剂点胶,由软件驱动,由纳米点胶设备执行。然后,这些细胞被播种在这些预填充的井中。

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