É necessária uma assinatura da JoVE para visualizar este conteúdo. Faça login ou comece sua avaliação gratuita.
High-Throughput DNA Plasmid Multiplexing and Transfection Using Acoustic Nanodispensing Technology
8,663 Views
•
13:27 min
•
August 08, 2019
DOI:
Summary
Automatically generated
This protocol describes high-throughput plasmid transfection of mammalian cells in a 384-well plate using acoustic droplet ejection technology. The time-consuming, error-prone DNA dispensing and multiplexing, but also the transfection reagent dispensing, are software-driven and performed by a nanodispenser device. The cells are then seeded in these prefilled wells.
Read Article
Cite this Article
Colin, B., Rocq, N., Deprez, B., Couturier, C. High-Throughput DNA Plasmid Multiplexing and Transfection Using Acoustic Nanodispensing Technology. J. Vis. Exp. (150), e59570, doi:10.3791/59570 (2019).
Copy