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High-Throughput DNA Plasmid Multiplexing and Transfection Using Acoustic Nanodispensing Technology
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August 08, 2019
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This protocol describes high-throughput plasmid transfection of mammalian cells in a 384-well plate using acoustic droplet ejection technology. The time-consuming, error-prone DNA dispensing and multiplexing, but also the transfection reagent dispensing, are software-driven and performed by a nanodispenser device. The cells are then seeded in these prefilled wells.
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Colin, B., Rocq, N., Deprez, B., Couturier, C. High-Throughput DNA Plasmid Multiplexing and Transfection Using Acoustic Nanodispensing Technology. J. Vis. Exp. (150), e59570, doi:10.3791/59570 (2019).
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