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Targeting Alpha Synuclein Aggregates in Cutaneous Peripheral Nerve Fibers by Free-floating Immunofluorescence Assay
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Targeting Alpha Synuclein Aggregates in Cutaneous Peripheral Nerve Fibers by Free-floating Immunofluorescence Assay

Targeting Alpha Synuclein Aggregates in Cutaneous Peripheral Nerve Fibers by Free-floating Immunofluorescence Assay

8,012 Views

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08:33 min

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June 25, 2019

DOI:

10.3791/59558-v

DOI:
10.3791/59558-v

•

08:33 min
June 25, 2019

•

7 Views
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1Laboratory for Biomedical Neurosciences,Neurocenter of Southern Switzerland, 2Neurology Department,Neurocenter of Southern Switzerland, 3Department of Neurology, Inselspital, Bern University Hospital,University of Bern, 4Faculty of Biomedical Sciences,Università della Svizzera Italiana

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Here, we present a protocol for a free-floating indirect immunofluorescence assay on skin biopsy sections that allows for the identification of disease specific conformation variants of alpha synuclein involved in Parkinson disease and multiple proteins of the peripheral nervous system.

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Parkinson’s DiseaseSkin BiopsyAlpha Synuclein AggregatesPeripheral Nerve FibersImmunofluorescence AssayCryoprotectantCryosectioningBiomarkerPathogenesis

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Vacchi, E., Pinton, S., Kaelin-Lang, A., Melli, G. Targeting Alpha Synuclein Aggregates in Cutaneous Peripheral Nerve Fibers by Free-floating Immunofluorescence Assay. J. Vis. Exp. (148), e59558, doi:10.3791/59558 (2019).

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