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Targeting Alpha Synuclein Aggregates in Cutaneous Peripheral Nerve Fibers by Free-floating Immunofluorescence Assay
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Targeting Alpha Synuclein Aggregates in Cutaneous Peripheral Nerve Fibers by Free-floating Immunofluorescence Assay
Capítulos
- 00:04Título
- 01:17Tissue Fixation and Storage
- 02:15Tissue Cut with a Cryotome
- 03:11Immunofluorescence Staining
- 05:34Immunofluorescence Imaging
- 06:01Results: Analysis of the Free-floating Immunofluorescence Assay
- 08:09Conclusion
Here, we present a protocol for a free-floating indirect immunofluorescence assay on skin biopsy sections that allows for the identification of disease specific conformation variants of alpha synuclein involved in Parkinson disease and multiple proteins of the peripheral nervous system.
