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Synthesis, Functionalization, and Characterization of Fusogenic Porous Silicon Nanoparticles for Oligonucleotide Delivery
Chapters
- 00:04Title
- 00:42Synthesis of Porous Silicon Nanoparticles (pSiNPs)
- 03:12Preparation of Fusogenic Lipid Film and Loading of siRNA in pSiNPs
- 04:20Coating siRNA-loaded pSiNPs with Fusogenic Lipids
- 05:42Conjugation of Targeting Peptides
- 06:45Results: Characterization of Fusogenic Silicon Nanoparticles for Oligonucleotide Delivery
- 08:16Conclusion
We demonstrate the synthesis of fusogenic porous silicon nanoparticles for effective in vitro and in vivo oligonucleotide delivery. Porous silicon nanoparticles are loaded with siRNA to form the core, which is coated by fusogenic lipids through extrusion to form the shell. Targeting moiety functionalization and particle characterization are included.
