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Synthesis, Functionalization, and Characterization of Fusogenic Porous Silicon Nanoparticles for Oligonucleotide Delivery
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Engineering
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JoVE Journal Engineering
Synthesis, Functionalization, and Characterization of Fusogenic Porous Silicon Nanoparticles for Oligonucleotide Delivery

Synthesis, Functionalization, and Characterization of Fusogenic Porous Silicon Nanoparticles for Oligonucleotide Delivery

DOI:
10.3791/59440-v

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08:53 min

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April 16, 2019

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,
1Materials Science and Engineering Program,University of California, San Diego, 2Department of Chemistry and Biochemistry,University of California, San Diego

Chapters

  • 00:04Title
  • 00:42Synthesis of Porous Silicon Nanoparticles (pSiNPs)
  • 03:12Preparation of Fusogenic Lipid Film and Loading of siRNA in pSiNPs
  • 04:20Coating siRNA-loaded pSiNPs with Fusogenic Lipids
  • 05:42Conjugation of Targeting Peptides
  • 06:45Results: Characterization of Fusogenic Silicon Nanoparticles for Oligonucleotide Delivery
  • 08:16Conclusion

Summary

Automatic Translation

Automatic Translation

We demonstrate the synthesis of fusogenic porous silicon nanoparticles for effective in vitro and in vivo oligonucleotide delivery. Porous silicon nanoparticles are loaded with siRNA to form the core, which is coated by fusogenic lipids through extrusion to form the shell. Targeting moiety functionalization and particle characterization are included.

Tags

Porous Silicon NanoparticlesFusogenic NanoparticlesSiRNA DeliveryGene SilencingIn Vivo Gene ModulationHydrofluoric Acid EtchingPorous Silicon SynthesisNanoparticle Characterization

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