Journal
/
Ingenieurwesen
/
Synthesis, Functionalization, and Characterization of Fusogenic Porous Silicon Nanoparticles for Oligonucleotide Delivery
JoVE Journal
Ingenieurwesen
Zum Anzeigen dieser Inhalte ist ein JoVE-Abonnement erforderlich.  Melden Sie sich an oder starten Sie Ihre kostenlose Testversion.
JoVE Journal Ingenieurwesen
Synthesis, Functionalization, and Characterization of Fusogenic Porous Silicon Nanoparticles for Oligonucleotide Delivery

Synthesis, Functionalization, and Characterization of Fusogenic Porous Silicon Nanoparticles for Oligonucleotide Delivery

DOI:
10.3791/59440-v

•

08:53 min

•

April 16, 2019

•

,
1Materials Science and Engineering Program,University of California, San Diego, 2Department of Chemistry and Biochemistry,University of California, San Diego

Kapitel

  • 00:04Titel
  • 00:42Synthesis of Porous Silicon Nanoparticles (pSiNPs)
  • 03:12Preparation of Fusogenic Lipid Film and Loading of siRNA in pSiNPs
  • 04:20Coating siRNA-loaded pSiNPs with Fusogenic Lipids
  • 05:42Conjugation of Targeting Peptides
  • 06:45Results: Characterization of Fusogenic Silicon Nanoparticles for Oligonucleotide Delivery
  • 08:16Conclusion

Summary

Automatische Übersetzung

Automatische Übersetzung

We demonstrate the synthesis of fusogenic porous silicon nanoparticles for effective in vitro and in vivo oligonucleotide delivery. Porous silicon nanoparticles are loaded with siRNA to form the core, which is coated by fusogenic lipids through extrusion to form the shell. Targeting moiety functionalization and particle characterization are included.

Tags

Porous Silicon NanoparticlesFusogenic NanoparticlesSiRNA DeliveryGene SilencingIn Vivo Gene ModulationHydrofluoric Acid EtchingPorous Silicon SynthesisNanoparticle Characterization

Article

Embed

ZUR WIEDERGABELISTE HINZUFÜGEN

Usage Statistics

Verwandte Videos

Optical Trapping of Nanoparticles

Synthesis and Functionalization of Nitrogen-doped Carbon Nanotube Cups with Gold Nanoparticles as Cork Stoppers

Monolayer Contact Doping of Silicon Surfaces and Nanowires Using Organophosphorus Compounds

Selective Area Modification of Silicon Surface Wettability by Pulsed UV Laser Irradiation in Liquid Environment

Synthesis and Functionalization of 3D Nano-graphene Materials: Graphene Aerogels and Graphene Macro Assemblies

Integration of Light Trapping Silver Nanostructures in Hydrogenated Microcrystalline Silicon Solar Cells by Transfer Printing

In Situ Synthesis of Gold Nanoparticles without Aggregation in the Interlayer Space of Layered Titanate Transparent Films

Enrich and Expand Rare Antigen-specific T Cells with Magnetic Nanoparticles

Procedure for the Transfer of Polymer Films Onto Porous Substrates with Minimized Defects

Method Development for Contactless Resonant Cavity Dielectric Spectroscopic Studies of Cellulosic Paper

Read Article