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单分子跟踪显微镜 - 确定细胞分子扩散状态的工具
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Single-Molecule Tracking Microscopy – A Tool for Determining the Diffusive States of Cytosolic Molecules
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单分子跟踪显微镜 - 确定细胞分子扩散状态的工具

7,979 Views

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10:21 min

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September 05, 2019

DOI:

10.3791/59387-v

DOI:
10.3791/59387-v

•

10:21 min
September 05, 2019

•

4 Views
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1Department of Chemistry,University of Virginia, 2Department of Molecular Physiology & Biological Physics,University of Virginia School of Medicine

Summary

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3D 单分子定位显微镜用于探测活细菌细胞中荧光标记蛋白质的空间位置和运动轨迹。本文描述的实验和数据分析方案根据集合的单分子轨迹确定细胞蛋白的普遍扩散行为。

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Cite this Article

Rocha, J. M., Gahlmann, A. Single-Molecule Tracking Microscopy - A Tool for Determining the Diffusive States of Cytosolic Molecules. J. Vis. Exp. (151), e59387, doi:10.3791/59387 (2019).

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